Potential of soil fungi to produce penicillin V acylase and its optimization conditions
نویسندگان
چکیده
منابع مشابه
Penicillin G Acylase, a biocatalyst and its potential application
The PGA enzyme belongs to the family of N-terminal nucleophile (Ntn) hydrolases. The enzyme Penicillin G Acylase (PGA) mainly produced from Penicillium chrysogenum, E.coli. The synthesis of PGA enzyme is depends on the physico-chemical parameters like carbon source, pH, temperature and media were optimized for higher production of enzyme. There are different methods of purification of PGA enzym...
متن کاملProduction of Penicillin Acylase.
The production of penicillin acylase by Escherichia coli Ny.I/3-67 has been increased by phenylacetic acid and phenoxyacetic acid, which themselves strongly inhibit the function of this specific enzyme. Other carbonic acids also increased penicillin acylase production, but to a lesser degree; they also weakly inhibited enzyme function. The production of this enzyme was effectively repressed wit...
متن کاملOverexpression of Penicillin V Acylase from Streptomyces lavendulae and 1 Elucidation of its Catalytic Residues
a Department of Biochemistry and Molecular Biology I, Faculty of Biology, University 7 Complutense of Madrid, Madrid, Spain; and b Department of Environmental Biology, Centro 8 de Investigaciones Biológicas, CSIC, Madrid, Spain 9 10 Running head: Approach to catalytic mechanism of Penicillin V acylase from Streptomyces 11 lavendulae 12 #Address correspondence to Isabel de la Mata, [email protected]...
متن کاملOptimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase
Penicillin G acylase from E. coli TA1 was immobilized by Cross-Linked Enzyme Aggregates (CLEA), a new method for immobilization. This biocatalyst and commercial immobilized penicillin G acylase (PGA-450) were used to study the effect of pH, temperature and substrate concentration on the synthesis of ampicillin from phenyl glycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA). Compare...
متن کاملOptimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase
Penicillin G acylase from E. coli TA1 was immobilized by Cross-Linked Enzyme Aggregates (CLEA), a new method for immobilization. This biocatalyst and commercial immobilized penicillin G acylase (PGA-450) were used to study the effect of pH, temperature and substrate concentration on the synthesis of ampicillin from phenyl glycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA). Compare...
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ژورنال
عنوان ژورنال: African Journal of Pharmacy and Pharmacology
سال: 2013
ISSN: 1996-0816
DOI: 10.5897/ajpp2013.3509